The Roles of Alpha-Momorcharin and Jasmonic Acid in Modulating the Response of Momordica charantia to Cucumber Mosaic Virus

نویسندگان

  • Ting Yang
  • Yao Meng
  • Li-Juan Chen
  • Hong-Hui Lin
  • De-Hui Xi
چکیده

Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein with a molecular weight of 29 kDa that is found in Momordica charantia, and has been shown to be effective against a broad range of human viruses as well as having anti-tumor activities. However, the role of endogenous α-MMC under viral infection and the mechanism of the anti-viral activities of α-MMC in plants are still unknown. To study the effect of α-MMC on plant viral defense and how α-MMC increases plant resistance to virus, the M. charantia-cucumber mosaic virus (CMV) interaction system was investigated. The results showed that the α-MMC level was positively correlated with the resistance of M. charantia to CMV. α-MMC treatment could alleviate photosystem damage and enhance the ratio of glutathione/glutathione disulfide in M. charantia under CMV infection. The relationship of α-MMC and defense related phytohormones, and their roles in plant defense were further investigated. α-MMC treatment led to a significant increase of jasmonic acid (JA) and vice versa, while there was no obvious relevance between salicylic acid and α-MMC. In addition, reactive oxygen species (ROS) were induced in α-MMC-pretreated plants, in a similar way to the ROS burst in JA-pretreated plants. The production of ROS in both ibuprofen (JA inhibitor) and (α-MMC+ibuprofen)-pretreated plants was reduced markedly, leading to a greater susceptibility of M. charantia to CMV. Our results indicate that the anti-viral activities of α-MMC in M. charantia may be accomplished through the JA related signaling pathway.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of salicylic acid and jasmonic acid on the symptoms of the disease and the expression of defense genes on a greenhouse cucumber infected with CMV

Cucumber is one of the most important vegetable and summer crops economically and is the fourth most cultivated plant in the world after tomatoes and potatoes. In this study, the effect of plant inducers including salicylic acid and jasmonic acid on the viral disease of greenhouse cucumber with Cucumber mosaic virus (CMV) and also the expression of defense genes was investigated. Treatments inc...

متن کامل

Influence of salicylic and jasmonic acids on the antioxidant systems of tomato (Solanum lycopersicum cv. Superchief) plants under biotic stresses. Sahar Gholi-Tolouie1, Mahdi Davari2, Nemat Sokhandan-Bashir1 and Mohammad Sedghi3*

Changes in the activity of antioxidant enzymes, proline, and total soluble carbohydrates (TSC) were evaluated in tomato (Solanum lycopercicum cv. Superchief) plants in response to salicylic acid (SA) and jasmonic acid (JA) and inoculation with CMV (Cucumber mosaic virus) on days 0, 1, 2, 4, 6, 8, and 15 after virus inoculation (dpi). Results showed that proline content increased in all treatmen...

متن کامل

A Novel Method for Simultaneous Production of Two Ribosome-Inactivating Proteins, α-MMC and MAP30, from Momordica charantia L

Alpha-momorcharin (α-MMC) and momordica anti-HIV protein (MAP30) from Momordica charantia L. have been confirmed to possess anti-tumor and anti-virus activities. Traditional purification methods of these two ribosome-inactivating proteins (RIPs) were separate which was time consuming and cost effective as well as low efficient. In order to obtain sufficient samples for researches, a strategy co...

متن کامل

Association of Tomato Leaf Curl New Delhi Virus, Betasatellite, and Alphasatellite with Mosaic Disease of Spine Gourd (Momordica dioica Roxb. Willd) in India

Background: Spine gourd (Momordica dioica Roxb. Willd) is one of the important cucurbitaceous crops grown across the world for vegetable and medicinal purposes. Diseases caused by the DNA viruses are becoming the limiting factors for the production of spine gourd reducing its potential yield. For the commercial cultivation of the spine gourd, propagation material used by most o...

متن کامل

Characterization of Cucumber Mosaic Virus From Mint in Tehran Province

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2016